which rep?

I would like to say out here that they fired, in a cowardly fashion might I add, our local sales rep. A man who went through a lot for Invitrogen and a good rep. Can't believe it. I certainly will be looking at other media and enzyme vendors. How can a manager can do this ?

matrix management, with open eyes
http://www.youtube.com/watch?v=P01ZxSb3SZM

Eh?

So how much does it *actually* cost to do a run on an IT machine? By that I mean all in, library prep ePCR, reagents, chip etc.

And are any of these 1Gb Chios actually in the wild yet. If so, do they yield a Gb of data?

And what is the quality compared to the earlier chips?

by shot up, you must mean QV17 from QV20. How quite dramatic...

read comment: I've seen some data from the Ion Torrent also (soon to be published). Quality and read length are certainly the most important area for them to improve, as the method (as of a month or two ago) was only getting about 67-long reads if you trimmed enough to get 1% error rates. The error rates shot up enormously towards the end of 100-long reads. I think that they can fix this in the next 6 months or so, and they are going to have to in order to stay competitive.

http://omicsomics.blogspot.com/2011/04/better-template-prep-for-ion-anno...

He won't have a link because it's not true. The system already does trimming as well, so no one post trims even more.

What's this about 60 base reads - I had a look at the Life website and could only find the press release - no technical information - do you have a link to where you saw this?

they are advertising it on the website, does anyone have plans to order a one touch sample prep machine???

I'm impressed with the fact that it doesn't exist yet and that the machine still needs reads to be trimmed to around 60 bp to have any value. Isn't the spec at 100 bp ? Can I return my machine, whatÈs the rule on that ?

I'm impressed at claims for the one touch sample prep for ion torrent....3 hours that's great...

GL doesnt have to sell to anyone but the shareholders.

Coming on here to ask for reasonable feedback of a product is a shit idea. There is nothing but trolls and disgruntled folk here. You should ask a real discussion group about product feedback.

LIFE'S a pissa
That's for sure
Working there?
Never a bore

But a family
Support you must
Not an employer
You can trust

Dr Doom

haha 36 samples a day, what are you talking about ? Who cares if your data is sh@t and the hands on time is a nightmare. You are perfect for Lifetech... Good luck with this... That machine is a nightmare and there are so many simple alternatives to it.

why? my manager wants to try it out since they are offering a really good deal on it 36 samples in one day...he wants me to try it.

That product is pure sh@t

Is anyone buying the new BT/LT consumable. The Open Array gene expression for miRNAs? Is it good, useful? Have they made any improvements to the machine?

I believe this is the worst thread ever in the history of the rumor mill. Winning ? I think not.

To all the high-fivers on Life Link Marketing Innovation who thought this partity was the cleverest thing ever...when LIFE actually comes up with a marketing campaign that has not already been run by Apple, or Google, or name any other internet/electronic company, THEN you can post it on "Marketing Innovation". If you are just copying other companies's sh!t, create a group for marketing rip offs and leave the innovation site to all the people that at least attempt to be original.

Life Tech is the Charlie Sheen of sequencing... WINNING!

http://www.youtube.com/watch?v=GUr17pHezUo&feature=channel_video_title

Eat that Illumina!

"Illumina have been lying and taking low blows for years.
I'm pretty sure the last accuracy flyer i received from ILMN had solid 2 data on it...in 2011."

Maybe if someone could actually get published with a later SOLiD system ILMN wouldnL't have to use SOLiD 3 data.

Let's recap all the truth from ABI - SOLiD PI, Starlight, 300 Gb on SOLiD 4, PE reads, simplifying ePCR (if 900+ steps to 400+ steps is your idea of simplification), fractional use flowcells (well not really since you can only use them once)...lots of honesty flowing out of ABI there

As an employee of LT, I know that I would never purchase an LT product unless I have no other choice. They cannot be trusted.

What does that ignorant reply think sample prep is?

With every sequencer you have to make a library. For a useful sequencer (HiSeq, not SOLiD) that library can go into a cBot for automated amplification on the flowcell. Effectively having the cBot also sequence is the MiSeq. The user experience is golden. You can try and make automated bridge amplification into sequencing sound like a bad thing. It shows you have never run a next gen sequencer. You must also think people love the EZ bead over the cBot.

You consider sample prep post library creation? The PGM experience can be a nightmare. Let me make plates and plates of emulsions for my 28 runs. What is that, at least 14 full 96-well plates that I have to prepare, run on the 5 hour PCR cycle (3 days or 70 hours of just PCR) , then spend hours breaking the emulsions and doing enrichment? All that just to get beads to load into the sequencer. I'd rather pipet in my library, hit start and go do more productive than being a Life emulsion bead slave.

Ignorant is thinking anyone would prefer that over simply loading a library into the sequencer. So what it takes the machine longer to sequence? At least it does millions of reads to the PGM's tens of thousands of reads. Illumina sequencing is a known quantity. Data off the PGM has not been peer reviewed to substantiate their crazy claims and stupid marketing schemes.

Hmm, cure cancer with emPCR and Q17? Sure sounds ignorant to me. The PGM is just a crappy 454 with better marketing. Good luck.

I think the rep really wanted that Rolex in a bad way... How cheezy is that ?

The rereply is totally ignorant. Sample prep is locked up on the Miseq during the run. Library prep is totally separate, and libraries are cake. Nextera takes forever to optimize anyways. There are much more reliable automation systems out there.

The Ion will be cranking out GB runs multiple times a day while the MiSeq is churning for 27hrs.

My Life Tech rep said that if I bought two PGMs now and they didn't hit their promised spec in six months when the MiSeq came out, that Life would take back my PGMs. They are confident they will blow away Illumina with both read length, output, and huge improvements in sample preparation and I totally believe they will.

I think the sample prep issue comment is totally ignorant.

You can make libraries for both instruments in parallel. Use Nextera for the Miseq and it can happen in 30 minutes.

Either way you need to make libraries independent of the machine. For the MiSeq, I can pipette in that library and hit go. The instrument does automate the cluster generation and sequencing but it is one smooth step.

The PGM takes the same library, and you do emulsification, emPCR, break the emulsions, enrich, bead count, huzzah! you now can pipet a sample into the machine and hit go.

Saying the MiSeq is locked up and you can't prep samples is again a very ignorant thing a Life Technology rep would tell a customer.

Actually, it about time LT marketing grew some balls. Illumina have been lying and taking low blows for years. why not see what LT can come up with? I'm pretty sure the last accuracy flyer i received from ILMN had solid 2 data on it...in 2011.

Because the you can do the sample prep while the PGM machine is running. And you can prepare PGM samples in parallel. The MiSeq sample prep is locked up when it is running.

Illumina MiSeq runs without needing to do emPCR will all get done faster than PGM runs that each take days to prep. This ad with the two machines * two runs a day * 7 days = "28 runs per week" = a few weeks of sample prep. I wonder if they can also sell me a used car?

Interesting approach from Life, though I think this shows how desperate they are to get their machine out there before the (presumably higher quality) Illumina alternative hits the market.

The whole Apple vs PC angle doesn't really work though. Why do people buy the more expensive Apples instead of PCs? Because they are pleasurable to use and look and feel great.

On the other hand, the IT machine looks terrible, while the Miseq I saw at AGBT looked gorgeous, and should support a hugely more pleasurable user experience, if Ilumina's previous product offerings are anything to go by.

If Life are not careful, stuff like this ad, and GL going on TV saying that the IT can sequence whole chunks of your genome (actually a small fraction of 1% of your genome would be more accurate) are going to backfire on them badly.

Back in the day, Life had a decent reputation for quality instruments. All of the iterations of Solid sequencers which never quite cut it in terms of workflow or data throughput, despite their outlandish claims, have not laid a great foundation for this sort of marketing.

Beware you don't look too desperate.......

Wow. Stay Classy Life Tech

Life Tech is the Charlie Sheen of sequencing... WINNING!

http://www.youtube.com/watch?v=GUr17pHezUo&feature=channel_video_title

Eat that Illumina!

http://news.morningstar.com/all/business-wire/20110406005820/gnubio-make...

yes, more synergies and consolidations are coming.

like I said. there is no move. get back to work.

What does Buba think of all this:hogwash man !
http://www.lifetechnologies.com/news-gallery/news-and-views/photos/Greg-...

so are they moving FC ?

that was such a hit has celera diagnostic... what's the link anyways behind pharma and genomics ? ZILTCH !

I think life has already thought about the next step. they don't just want to a tools company, they're trying to become a biopharmaceutical (tech + pharma)

Well clearly the latest news from AACR are showing some reality check and some restraint on this scam "Cancer genomes are extraordinarily complicated," Ellis said in a statement, adding the result of his and his colleagues research "explains our difficulty in predicting outcomes and finding new treatments."

Hey Greg, how about you start giving some respect to reality.

The problem here is that scientists are at the mercy or their own cheap talk. Some of them in bed with the notion of bad gene=cancer. Ya there is thousands of mutation shifts per cell per mass per environment per genome. It's like the mightiest of all mighty Rubik cube, except it keeps changing before you change one piece.

Imagine the same billion dollar invested in exercise VS less sugar, meat, salt and TV.

I challenge any frigging molecular cancer genetics on that premise. I can save more lives now with clean water than a million sequencers will in 10 years.

The model is flawed.

So eventually we know everyones' genome and every gene for every type of cancer and genetic disease. Fine, I'll accept that. And then . . . what? Ever think of what you would need to do after that? Perhaps some drug discovery and/or regenerative medicine? Or at least gaining an understanding of the patient and disease on a level BEYOND the gene? In other words, maybe needing applications that Life Tech has basically taken for granted lately, if not treated with outright disdain?

I guess this is a case study in what happens when people with only the vaguest whiff of a scientific background decide they want to run a great big biotech. The ideas are well-considered but fundamentally flawed because their originators don't know as much as they think or would like. Same idea--someone can put a lot of work into building a table, but if they don't understand woodworking and the people who would need the table then the table is going to suck and people will dislike it. And if this person is greedy, they then try to sell this crap table to a person for the same price as a premium piece of furniture.
Same idea as, say, some of the instruments we've been seeing from Life.

SO to recap--this looks like a case in which arrogant pricing and business tactics meet flawed understandings of current science and the actual marketplace. It's GREAT to be an outside observer of this, an example of a young industry learning by making big mistakes.

Sequencing everyone's genome will be $1.

Will that be for each genome, or do you think the Ion Torrent will get that down to $1 for everyone? LIFE is claiming Lucifer's law (sequencing corollary to Moore's law) is 10x increase in throughput every 6 months. So we should be down to a penny a genome by 2014...

I don't care if you sequence everything in the world, ion torrent won't solve 100% of the world's problems...

Sequencing everyone's genome will be $1. Life Technologies will go bankrupt because of altruism. After everyone sees their genome, they get off their fat a$$ and exercise, improving global health.

GL returns to his home planet, job well done.

Patenting genes for cash and beating cancer with an ion torrent... Good one, there is still people dying from dehydration and this clown is saying that sequencing a genome will give us THE ANSWER to cancer...

Give us a sample.

The amount of bullshit coming out of GL's mouth at the Clinton initiative is mindblowing ! What on earth is that loser doing there... ridigigilous ! He is worst than a bank CEO !

nearly nonstop layoffs in Eugene