What's going on at LIFE?

Synergies=Layoffs
coming B4 year end.

Do tell us the details of

Do tell us the details of your analysis - oh go on - please do....

Same dataset, CLC, my

Same dataset, CLC, my analysis is well over your claims. Time to read a manual.

It's actually delivering

It's actually delivering exactly what they said it would, and way way more in the hands of decent users.

So where is this data and who are these decent users? Kudos to LIFE for making their E. coli data public without registration restrictions etc., but those 8 runs on the IT to generate just over a million reads must be close to state of the art right now. LIFE must be one of the decent users. That dataset just isn't very much better than the BGI 7 run set, so BGI must also be on the list of decent users. Likewise with Edge Bio, since LIFE has certified them as a service provider.

I've assembled datasets (CLC Workbench, since there is little or no guidance out there on k-mer size for Velvet) from all these sources and have not be overly impressed. With a contig length cutoff of 200, the latest datasets generate thousands of contigs for E. coli, where nearly half are less than 1000 bases in length. The consensus sequences have gaps every 5-10 bases, since more than half the reads have insertions or deletions relative to the consensus.

If this was the first next-gen datasets the world had ever seen, they would be impressive. But state of the art today with E. coli sized genomes is being able to set your contig cutoff at 5000 bases, and easily generate contigs 20x the size the IT is generating.

"The good news is the job

"The good news is the job market is picking up and all the good performers can sing a synonmyous "F YOU" to LIFE and their 3% raises."

This year's raises were more in the 1-2% range. That's less than the inflation rate.

"It chaps their a$$ that the

"It chaps their a$$ that the company they hate so much is doing well and none of their doomsday predictions have come true."

Anybody who thinks that LIFE is doing well clearly does not see the daily sales report. GL barely pulled Q1's 1% growth out of his a$$. I'd have more confidence that LIFE genetically engineered the Nazi E Coli strain purposely to get some growth for Q2 - worked so well with H1N1,let's try again. Otherwise the numbers are looking PATH-E-TIC. Losing market share in core markets faster than an amputee can bleed out!

The good news is the job market is picking up and all the good performers can sing a synonmyous "F YOU" to LIFE and their 3% raises. The mass exodus has begun!!!! We are hiring Ce-Lo to give GL a private performance on the way out!

It's actually delivering

It's actually delivering exactly what they said it would, and way way more in the hands of decent users.

Correct. There is now plenty

Correct. There is now plenty of data out there, and it is disappointing data. Nothing to do with it being Life or Roche or Illumina. Life have spun a lot of expectation, and delivered small numbers of dreadfully poor quality short reads. I personally have nothing against Life, just the ridiculous hype they have indulged in regarding Ion Torrent, in an attempt to try and capture some market share.

the low accuracy claims are a

the low accuracy claims are a little old. there is plenty of data out there now.

Correct - but the pathetic idiots on this rumor board desperately want Life to fail.

It chaps their a$$ that the company they hate so much is doing well and none of their doomsday predictions have come true.

Look around on this site - it seems that no company is up to the high standards of the Biofind brethren...especially Lifetech. Maybe they should all band together and form some kind of Utopian super company! But...that would probbly take too much time away from the bile and rumor mongering that seems to fill the void of their desperate lives.

Carry on.

the low accuracy claims are a

the low accuracy claims are a little old. there is plenty of data out there now.

The market needed to be

The market needed to be completed:

Large number of short, accurate reads = Illumina

Moderate number of moderate length, accurate reads = 454

Small number of low accuracy, very long reads = Pac Bio

Small number of low accuracy, very short reads = Ion Torrent

5- R&D support towards making

5- R&D support towards making and then keeping IT a viable player.

1- cannibalizing CE market 2-

1- cannibalizing CE market
2- Selling very low price
3- High support requirements
4- At this rate it would take you 7 years to pay your investment back

There is nothing wrong with IT but Life is making it look bad by all possible means.

growth, yes....managing the

growth, yes....managing the growth is another story.

i read this blog the other

i read this blog the other day
the writer said to invest in LT and IT because it has more growth

in their words ILMN has reached maturity with its technology

I think the writer makes a good point.

shipped a few? you mean a few

shipped a few? you mean a few hundred...

Shipping them amongst yourselves doesn't count...

Great, a few hundred could

Great, a few hundred could produce as much data as a single HiSeq. Kudos to LT.

shipped a few? you mean a

shipped a few? you mean a few hundred...

It's now like Illumina didn't

It's now like Illumina didn't have problems with their GAIIX and HiSeq. Our first GAIIX didn't work for 6 months and then they replaced it with a new instrument.

They shipped a few and cannot

They shipped a few and cannot support them. Kit shortages, lack of trained FAS and FSE staff. They oversold their abilities and with users having so much trouble, they need to hide in the background and whimper until they improve it enough to be a viable instrument or move the team back to Starlight/SOLiD or give up the sequencing market and focus on something else in the Life Tech Portfolio.

Well I always liked the PC

Well I always liked the PC guy better than the Mac guy, who seemed a snooty little sheet.

Interesting that those

Interesting that those YouTube films with the MiSeq vs PGM guy have stopped. Could it possibly be that now actual ion data is out there in the wild, people can see just how poor it is, and even the hubris displayed by Mr Rothberg and Life cannot justify any further You Tube fun? Or maybe they got feedback that people quite liked the Miseq guy?

By major workforce reduction,

By major workforce reduction, I here a wave is coming in the next few days.

mkt cap of Life decrease with

mkt cap of Life decrease with an almost $700M purchase of another company with little sales? How will it work if IT doesn't make milestones?

ILMN is a well run company so

ILMN is a well run company so the mkt cap relfect that. No surprise here.

Market cap of ILMN just about

Market cap of ILMN just about to surpass that of LIFE according to Google Finance. Had not expected this tipping point to be reached until Q4 2011.

Kudos to GL and KM and the

Kudos to GL and KM and the rest.

GL dumped KM awhile back. The Chief Scientific Officer also left to head up the ATCC.

If my reading is correct,

If my reading is correct, the following analysis of data on new E. coli outbreak strain generated from Ion Torrent runs do indicate a sequencing issue with homopolymers despite a lack of disclosure about Ion Torrent real data quality.

http://bacpathgenomics.wordpress.com/2011/06/04/ehec-genomes/

With the same chemistry, MiSeq may more likely have similar performance as HiSeq. Can not wait to try MiSeq.

I am anxious to see how the

I am anxious to see how the MiSeq pans out. I would definitely favor it if I get the same data quality as the HiSeq but an overnight run time.

Ditto. Lots of people waiting on the sidelines right now. I'm not seeing these latest Ion Torrent results being even equivalent to a 454 Junior.

Why do you keep reposting

Why do you keep reposting this? No one cares about LIFE Technologies.

7 runs, almost $10K. It may

7 runs, almost $10K. It may be fast, but you pay for it both in price and data quality.

I am anxious to see how the MiSeq pans out. I would definitely favor it if I get the same data quality as the HiSeq but an overnight run time.

Terrible data quality,

Terrible data quality, terrible assembly.

For the LIFE data, it's hard to judge the quality, since they haven't released it. Only an "assembly" where they bootstrapped the process by aligning it to existing E. coli genomic sequence(s) and then doing de novo assembly of anything that didn't fit. That still resulted in more than 350 contigs. The Chinese data (done on Ion Torrent) isn't great. 7 runs to get 12 x coverage, de novo assembly into > 3000 contigs.

Three days with library/bead

Three days with library/bead prep. Three hours from prepared instrument start to finish. Terrible data quality, terrible assembly.

I really admire all these

I really admire all these really smart people at LIFE Technologies. From the dumbest to the smartest. They are all brilliant. Even the dumbest CEO. To the smartest dishwarsher...

No, it is human shit. I

No, it is human shit. I think it is human shit.

LIFE has aan opportunity to

LIFE has aan opportunity to make a real difference this time. Kudos to GL and KM and the rest. Here's a chance for them to shine.

"Life Technologies

"Life Technologies Corporation today announced that whole genome data from DNA sequencing samples from all 1,605 people that have that E. coli strain on the Ion Personal Genome Machine (PGM™) proves that the bacterium that caused deadly outbreak in Germany is a new hybrid of pathogenic E. coli strains. Using 25 of the revolutionary instruments, all 1,605 genomes were sequenced in only three days."

Bullshit.

Life Technologies Corporation

Life Technologies Corporation today announced that whole genome data from DNA sequencing samples from all 1,605 people that have that E. coli strain on the Ion Personal Genome Machine (PGM™) proves that the bacterium that caused deadly outbreak in Germany is a new hybrid of pathogenic E. coli strains. Using 25 of the revolutionary instruments, all 1,605 genomes were sequenced in only three days.

"scientists completed a whole

"scientists completed a whole genome sequencing run"

They did not sequence a genome, they "completed a whole genome sequencing run". There is a difference, and LIFE uses very precise language in their press releases. They put a whole genome on the machine and completed the run. They found some genes that indicated a hybrid E. coli species.

the (original) press release

the (original) press release said three days...

The press release is very

The press release is very clear:

When a deadly strain of never-before-seen E. coli broke out in Germany, speed to results became the top priority. With the Ion PGM™ sequencer, scientists completed a whole genome sequencing run and gained life-saving insights when they needed them the most—and they did it in just 2 hours.

It worked for them—imagine what the Ion PGM™ could do for your research

You just don't know how to

You just don't know how to read a LIFE press release. It just said they did genomic sequencing and found some genes, not that they actually sequenced a genome...

It took at least 5 touches,

It took at least 5 touches, as they needed 5 whole runs to get enough data just to sequence 1 e coli bug. But of course they will have 1 Gb per run with high quality 200 base reads and 1 hour sample prep by year end so there is no need to worry about the performance of your PGM......

They did the same thing

They did the same thing during the h1n1 outbreak.

it was single molecule and

it was single molecule and one of the IT chips sequenced every strain at once. for $5. no need for sample prep, it is a one touch of the magic wand to get the sample on the instrument.

Now Life is exploiting the e.

Now Life is exploiting the e. coli outbreak, claiming IT systems ( in China and Germany) sequnced the strain in three hours. Does that include sample prep? Sleazy...

Who has a SOLiD 5500 series?

Who has a SOLiD 5500 series? How many sold have actually been delivered? How many orders were never made due to having no product? SOLiD is dead. Arrogance wouldn't have saved it.

All the old arrogant ABI

All the old arrogant ABI people are at Illumina and PacBio. The shift in arrogance is quite clear.

I am hearing some serious

I am hearing some serious slashing at LIFE as the numbers have no where to go for this quarter, everything frozen solid (ah!)

I spend a very long time on

I spend a very long time on hold today with life technolgies customer service until I realized they are doing what ABI use to be.

Why do you keep posting this?

Why do you keep posting this? Isn't that guy long gone. Your persistence in voicing this accusation has been taken as an indictment of the current CEO Mr. Taterhead. That isn't fair is it?

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