With the expertise and accumulated experience, Creative Biomart is much honored to briefly introduce the importance of protein and protein purification:
The most important reason that researchers study proteins and their function is try to understand both cells and organisms in the context of biology. Here the following lists some of such reasons:
They serve as catalysts that maintain metabolic processes in the cell;
They serve as structural elements both within and outside the cell;
They are signals secreted by one cell or deposited in the extracellular matrix that are recognized by other cells;
They are receptors that convey information about the extracellular milieu to the cell;
They serve as intracellular signaling components that mediate the effects of receptors;
They are key components of the machinery that determines which genes are expressed and whether mRNAs are translated into proteins;
They are involved in manipulation of DNA and RNA through processes such as: DNA replication, DNA recombination, RNA splicing or editing.
People spend much time and effort to achieving protein purification. In addition to the reason as protein do, they also include:
To clearly established that a particular biological activity (enzymatic activity, signaling capacity, etc.) actually resides in a unique protein.
To serve as extremely valuable biochemical reagents.
To study its enzymology, understand its affinity for particular substrates, or dissect its ability to catalyze enzymatic reactions.
To allow bio-organic chemists to modify specific residues to help understand how these residues confer particular structures or allow the protein to operate as catalyst.
Often be identified on the basis of fragments generated by ionizing the proteins or peptides derived from them and performing mass spectroscopy.
With the advent of site-directed mutagenesis and expression systems, it is possible to produce and purify a protein with essentially any mutation of interest. This allows the testing of a broad spectrum of hypotheses as well the design of "improved" protein molecules.
Using genetic engineering, it is possible to create novel proteins or combination of protein elements that can serve a specific function.
To help understanding the structure-function relationships of proteins by designing specific reagents that can be used to test the function of a protein in vivo.
Understanding 3-D protein structure has begun to allow us to understand how protein folding is controlled and it has provided a remarkable insight into the way the proteins act in vivo. The ability to visualize an ion channel, even at low resolution, must forever modify the way a scientist understands ion flow through the membranes.
Determining new protein structure is a major undertaking. For crystallography, protein crystals* must be made and defraction information must be analyzed.
To help purify a nucleic acid of interest.
About Creative Biomart
Creative Biomart is a global full-service biotechnology company with profound expertise and experience in offering recombinant proteins, antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes, Elisa Kits, biosimilar cell lines, transfected stable cell lines etc. to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. Our full-service covers Protein Expression (Bacterial Expression Systems (E. coli / Bacillus), Yeast Expression Systems (P pastoris / S cerevisiae), Baculovirus-insect Cell Expression Systems, Mammalian Expression Systems (CHO / 293)), Membrane Protein (Custom Membrane Protein Production, Cell Line Development and Membrane Preparation, Custom Lipoparticle Containing Membrane Protein, Lipid-based Protein Immobilization), PEGylation Services, Directed Evolution, Protein Engineering, Mutagenesis Services (Site-Directed Mutagenesis, Site-Saturation Mutagenesis, Controlled Randomization Service), Protein Microarray , Custom cDNA clone etc.
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